Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 82
Page 8-30
... vector in the reaction to achieve an optimal ratio of primer to template . • The final cDNA clone is bounded on both sides by complementary homopolymers ( dA : dT at the 3 ' terminus of the coding strand and dC : dG at the 5 ' terminus ) ...
... vector in the reaction to achieve an optimal ratio of primer to template . • The final cDNA clone is bounded on both sides by complementary homopolymers ( dA : dT at the 3 ' terminus of the coding strand and dC : dG at the 5 ' terminus ) ...
Page 8-39
... vector in the correct orientation with respect to the lacZ promoter , and only one in three of the molecules inserted in the right orientation will be in the correct reading frame to generate a fusion protein . Furthermore , because ...
... vector in the correct orientation with respect to the lacZ promoter , and only one in three of the molecules inserted in the right orientation will be in the correct reading frame to generate a fusion protein . Furthermore , because ...
Page 13-21
... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target DNA and the vector are similar in size and cannot be separated by gel electrophoresis , try to find a restriction ...
... vector sequences by digestion with restriction enzymes and polyacrylamide or agarose gel electrophoresis . If the target DNA and the vector are similar in size and cannot be separated by gel electrophoresis , try to find a restriction ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml