Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-38
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris Cl ( pH 8.0 ) , and combine the two ...
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris Cl ( pH 8.0 ) , and combine the two ...
Page 11-42
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
Page 13-31
... volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme ... volume of 2 μl ) ; B = 100 ng of linearized , phosphatase - treated vector ( in a volume of 2 μl ) ; and C = 100 ng ...
... volume of 100 μl , digest 10 μg of vector DNA ( phagemid or bacteriophage M13 replicative form DNA ) with an enzyme ... volume of 2 μl ) ; B = 100 ng of linearized , phosphatase - treated vector ( in a volume of 2 μl ) ; and C = 100 ng ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml