Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 58
Page 11-39
... wash . 2. Dilute the radiolabeled sample to 1.5 ml with H2O , and apply the whole sample to the column through the syringe . 3. Wash the Sep - Pak column with the following four solutions . Repeat step 1c after each wash . 10 ml of 25 ...
... wash . 2. Dilute the radiolabeled sample to 1.5 ml with H2O , and apply the whole sample to the column through the syringe . 3. Wash the Sep - Pak column with the following four solutions . Repeat step 1c after each wash . 10 ml of 25 ...
Page 12-19
... Wash the filters for 10 minutes in each of the buffers below in the order given . Transfer the filters individually from one buffer to the next . Use 7.5 ml of each buffer for each 82 - mm filter and 15 ml for each 138 - mm filter . TNT ...
... Wash the filters for 10 minutes in each of the buffers below in the order given . Transfer the filters individually from one buffer to the next . Use 7.5 ml of each buffer for each 82 - mm filter and 15 ml for each 138 - mm filter . TNT ...
Page 13-83
... wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and allow the pellet to ...
... wash and check the amount of radioac- tivity in both the pellet and the wash with a hand - held minimonitor before the ethanol is discarded . 4. After the final wash , carefully remove the ethanol by aspiration , and allow the pellet to ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml