Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 71
Page 8-61
... yield of first strand is usually close to 50 % of the weight of the poly ( A ) RNA in the reaction mixture . Notes i . An alternative procedure to monitor synthesis of the first strand of cDNA is to use a small amount of [ a - 32P ] ...
... yield of first strand is usually close to 50 % of the weight of the poly ( A ) RNA in the reaction mixture . Notes i . An alternative procedure to monitor synthesis of the first strand of cDNA is to use a small amount of [ a - 32P ] ...
Page 8-75
... yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the efficiency of cloning of cDNA ( plaque - forming units per ...
... yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the efficiency of cloning of cDNA ( plaque - forming units per ...
Page 10-20
... yield of the radioactive strand synthesized by the Klenow fragment of E. coli DNA polymerase I are affected by the relative concentrations of primer , template , and dNTPs . The Km of the enzyme is different for each of the four dNTPs ...
... yield of the radioactive strand synthesized by the Klenow fragment of E. coli DNA polymerase I are affected by the relative concentrations of primer , template , and dNTPs . The Km of the enzyme is different for each of the four dNTPs ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml