Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 76
Page 1-68
... μg / ml . 2. Set up the ligation mixtures as follows : a . Transfer 0.1 μg of the vector DNA to a sterile microfuge tube . Add an equimolar amount of foreign DNA . b . Add H2O to 7.5 μl , and warm the solution to 45 ° C for 5 minutes to ...
... μg / ml . 2. Set up the ligation mixtures as follows : a . Transfer 0.1 μg of the vector DNA to a sterile microfuge tube . Add an equimolar amount of foreign DNA . b . Add H2O to 7.5 μl , and warm the solution to 45 ° C for 5 minutes to ...
Page 2-88
... μg or more of the bacteriophage A vector DNA as described in steps 1-7 on page 2.83 . After step 7 of the procedure , adjust the concentration of the DNA with TE ( pH 8.0 ) to 250-500 μg / ml and reanneal the arms as described in step 2 ...
... μg or more of the bacteriophage A vector DNA as described in steps 1-7 on page 2.83 . After step 7 of the procedure , adjust the concentration of the DNA with TE ( pH 8.0 ) to 250-500 μg / ml and reanneal the arms as described in step 2 ...
Page 4-48
... ml in H2O ) to a final concentration of 70 μg / ml . Continue incubation for a further 14-18 hours at 37 ° C . The yield of virus particles containing single - stranded copies of the plasmid can be measured by infecting MV1184 ( see ...
... ml in H2O ) to a final concentration of 70 μg / ml . Continue incubation for a further 14-18 hours at 37 ° C . The yield of virus particles containing single - stranded copies of the plasmid can be measured by infecting MV1184 ( see ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml