Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 55
Page 2-28
... Sall 34590 Sall Smal 34070 Sall 36140 San 35150 EcoRI 35140 Sacl 35130 Xbal 35120 Sall 33100 Sal 35100 HindIII 35090 BamHI 29810 Sai 29750 Bgl 28790 BamHI 27250 Smal 26450 San 25740 Sacl 24970 Sacl 24190 Bgl 23990 EcoRI 23080 BamHI ...
... Sall 34590 Sall Smal 34070 Sall 36140 San 35150 EcoRI 35140 Sacl 35130 Xbal 35120 Sall 33100 Sal 35100 HindIII 35090 BamHI 29810 Sai 29750 Bgl 28790 BamHI 27250 Smal 26450 San 25740 Sacl 24970 Sacl 24190 Bgl 23990 EcoRI 23080 BamHI ...
Page 2-33
... Sall or EcoRI . Recombinants are Spi and thus can be selected on a P2 - lysogenic host . EMBL3A carries two amber ... Sall BamHI EcoRI In EMBL3 and EMBL3A , the polycloning sites and stuffer are as shown in the EMBL3A map : Sall , BamHI ...
... Sall or EcoRI . Recombinants are Spi and thus can be selected on a P2 - lysogenic host . EMBL3A carries two amber ... Sall BamHI EcoRI In EMBL3 and EMBL3A , the polycloning sites and stuffer are as shown in the EMBL3A map : Sall , BamHI ...
Page 2-45
... Sall and then ligating the bacteriophage in the presence of an oligonucleotide that does not reconstruct the Sall sites . This alteration causes the vector to become red gam . The resulting small - plaque phenotype was altered by ...
... Sall and then ligating the bacteriophage in the presence of an oligonucleotide that does not reconstruct the Sall sites . This alteration causes the vector to become red gam . The resulting small - plaque phenotype was altered by ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml