Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 24
Page 1-40
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 6. Remove the supernatant by aspiration . Dissolve the pellet of plasmid DNA in 400 μl of TE ( pH 8.0 ) . Extract the solution once with phenol , once with phenol ...
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 6. Remove the supernatant by aspiration . Dissolve the pellet of plasmid DNA in 400 μl of TE ( pH 8.0 ) . Extract the solution once with phenol , once with phenol ...
Page 4-29
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 5. Carefully remove all of the supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual ...
... centrifugation at 12,000g for 5 minutes at 4 ° C in a microfuge . 5. Carefully remove all of the supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual ...
Page 7-9
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Carefully remove the supernatant by aspiration . d . Redissolve the pellet in 300 μl of TE ( pH 7.6 ) , and add 1 ml of ethanol . Store the preparation at -70 ° C until ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Carefully remove the supernatant by aspiration . d . Redissolve the pellet in 300 μl of TE ( pH 7.6 ) , and add 1 ml of ethanol . Store the preparation at -70 ° C until ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml