Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page v
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
Page vii
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
Page xxii
... CLONING OF DOUBLE - STRANDED cDNA 8.21 Homopolymeric Tailing 8.21 Synthetic DNA Linkers and Adapters 8.23 Alternative Methods of Cloning cDNA 8.27 mRNA - CDNA CLONING 8.27 SEQUENTIAL ADDITION OF DIFFERENT LINKERS 8.27 CDNA CLONING IN ...
... CLONING OF DOUBLE - STRANDED cDNA 8.21 Homopolymeric Tailing 8.21 Synthetic DNA Linkers and Adapters 8.23 Alternative Methods of Cloning cDNA 8.27 mRNA - CDNA CLONING 8.27 SEQUENTIAL ADDITION OF DIFFERENT LINKERS 8.27 CDNA CLONING IN ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml