Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xix
... GELS 6.46 " Crush and Soak " Method 6.46 Other Types of Gels 6.49 STRAND SEPARATING GELS 6.49 DENATURING GRADIENT POLYACRYLAMIDE GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xix 6 Gel Electrophoresis of.
... GELS 6.46 " Crush and Soak " Method 6.46 Other Types of Gels 6.49 STRAND SEPARATING GELS 6.49 DENATURING GRADIENT POLYACRYLAMIDE GELS 6.49 7 PULSED - FIELD GEL ELECTROPHORESIS 6.50 Design of the Contents xix 6 Gel Electrophoresis of.
Page 6-8
... chambers . This is important when RNA is analyzed by electrophoresis . PREPARATION AND EXAMINATION OF AGAROSE GELS Preparation of an Agarose 6.8 Gel Electrophoresis of DNA Apparatuses Used for Agarose Gel Electrophoresis 6 8.
... chambers . This is important when RNA is analyzed by electrophoresis . PREPARATION AND EXAMINATION OF AGAROSE GELS Preparation of an Agarose 6.8 Gel Electrophoresis of DNA Apparatuses Used for Agarose Gel Electrophoresis 6 8.
Page
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml