Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-8
... host . The choice between the lytic and lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by ...
... host . The choice between the lytic and lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by ...
Page 2-43
... host is used for propagation and screening . In a nonsuppressing host , the Sam100 mutation prevents cell lysis and allows intracellular accumulation of the desired fusion protein . The bac- teriophage carries red and gam and thus can ...
... host is used for propagation and screening . In a nonsuppressing host , the Sam100 mutation prevents cell lysis and allows intracellular accumulation of the desired fusion protein . The bac- teriophage carries red and gam and thus can ...
Page 4-12
... HOSTS FOR BACTERIOPHAGE M13 VECTORS Because M13 enters the host cell through sex pili encoded by an F factor , only male bacteria are used to propagate the virus . ' Bacterial strains carrying F ' episomes and a number of genetic ...
... HOSTS FOR BACTERIOPHAGE M13 VECTORS Because M13 enters the host cell through sex pili encoded by an F factor , only male bacteria are used to propagate the virus . ' Bacterial strains carrying F ' episomes and a number of genetic ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml