Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 11
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... immunoprecipitation . See Immunopre- cipitation solid - phase radioimmunoassay , 18.19- 18.20 using two antibodies , 18.21-18.23 western blotting . See Western blotting Immunological screening of bacterial colonies , 12.21-12.28 lysis ...
... immunoprecipitation . See Immunopre- cipitation solid - phase radioimmunoassay , 18.19- 18.20 using two antibodies , 18.21-18.23 western blotting . See Western blotting Immunological screening of bacterial colonies , 12.21-12.28 lysis ...
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... immunoprecipitation enzymatic , 6.55 , 18.36-18.37 mechanical , 18.35 rapid ( RIPA buffer ) , 18.38 Yeast artificial chromosome ( YAC ) , ge- nomic DNA libraries , 9.5-9.6 YK537 , E. coli strain , genotype and uses , A.12 YT medium ...
... immunoprecipitation enzymatic , 6.55 , 18.36-18.37 mechanical , 18.35 rapid ( RIPA buffer ) , 18.38 Yeast artificial chromosome ( YAC ) , ge- nomic DNA libraries , 9.5-9.6 YK537 , E. coli strain , genotype and uses , A.12 YT medium ...
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... immunoprecipitation , 18.26–18.29 Si nuclease , activity and uses , 5.78-5.79 buffer , 4.43 , B.28 determining size of inserts cloned into bacteriophage M13 vectors , procedure , 4.43 digestion of hairpin loop , in cDNA synthesis , 8.15 ...
... immunoprecipitation , 18.26–18.29 Si nuclease , activity and uses , 5.78-5.79 buffer , 4.43 , B.28 determining size of inserts cloned into bacteriophage M13 vectors , procedure , 4.43 digestion of hairpin loop , in cDNA synthesis , 8.15 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml