Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 81
Page 1-54
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation recombinant plasmids may carry tandem ...
... inserted in only one orientation within recombinant plasmid restriction sites at junctions between plasmid and foreign DNAs are usually conserved foreign DNA can be inserted in either orientation recombinant plasmids may carry tandem ...
Page 1-87
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp ' gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
... insertion of a segment of rat pre- proinsulin cDNA into the PstI site of pBR322 did not inactivate the amp ' gene . Presumably , a small piece of foreign DNA had been inserted that did not alter the reading frame of the amp ' gene , so ...
Page 4-33
... inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector is cleaved with a single ...
... inserted into bacteriophage M13 vectors in exactly the same way that they would be inserted into a conventional plasmid . Four different methods are commonly used : 1. The polycloning site of the vector is cleaved with a single ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml