Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 51
Page 1-23
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
Page 1-56
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
Page 4-33
... linear DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the ... linear molecules with incompatible termini ( see Chapter 1 , pages 1.53-1.56 ) . The linear vector is ligated to an ...
... linear DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the ... linear molecules with incompatible termini ( see Chapter 1 , pages 1.53-1.56 ) . The linear vector is ligated to an ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml