Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-8
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
Page 2-95
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
Page 2-98
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml