Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxiv
... PURIFICATION 17.37 Inclusion Bodies 17.37 CELL LYSIS 17.38 PURIFICATION AND WASHING OF INCLUSION BODIES : METHOD 1 17.39 PURIFICATION AND WASHING OF INCLUSION BODIES : METHOD 2 17.40 SOLUBILIZATION OF INCLUSION BODIES References 17.42 ...
... PURIFICATION 17.37 Inclusion Bodies 17.37 CELL LYSIS 17.38 PURIFICATION AND WASHING OF INCLUSION BODIES : METHOD 1 17.39 PURIFICATION AND WASHING OF INCLUSION BODIES : METHOD 2 17.40 SOLUBILIZATION OF INCLUSION BODIES References 17.42 ...
Page 1-21
... Purification of plasmid DNA Growth of the Bacterial Culture Plasmids are almost always purified from cultures ( grown in liquid medium containing the appropriate antibiotic ) that have been inoculated with a single bacterial colony ...
... Purification of plasmid DNA Growth of the Bacterial Culture Plasmids are almost always purified from cultures ( grown in liquid medium containing the appropriate antibiotic ) that have been inoculated with a single bacterial colony ...
Page 2-85
... Purified arms of several of the most popular vectors ( e.g. , EMBL3 and 4 , Agt10 , Agt11 ) are available from ... Purification of Bacteriophage λ Arms 2 85 CENTRIFUGATION THROUGH SUCROSE DENSITY GRADIENTS 2 85.
... Purified arms of several of the most popular vectors ( e.g. , EMBL3 and 4 , Agt10 , Agt11 ) are available from ... Purification of Bacteriophage λ Arms 2 85 CENTRIFUGATION THROUGH SUCROSE DENSITY GRADIENTS 2 85.
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml