Antibody Engineering

Front Cover
Carl A. K. Borrebaeck
Oxford University Press, 1995 - Medical - 390 pages
As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.

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Contents

Antibody Structure and Function
3
Human Monoclonal Antibodies from VGene Repertoires
53
Synthetic Antibodies
89
Engineering the Antibody Combining Site by CodonBased
117
Human Metaphoric Antibodies from a Phagemid Library
133
Stragies for Humanizing Antibodies
159
SingleChain Fv Design and Production by Preparative Folding
185
Expressing Antibodies in Escherichia Coll
229
Antibodies and Antibody Fusion Proteins
267
The Cloning of Hybridoma V Regions for Their Ectopic
295
Immunization
330
Working Examples 345 Working Examples
346
The Integrated Vector System
353
The Expression of Single Chain Antibodies
363
Copyright

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Page 358 - Gossen, M. and Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Page 375 - An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46: 291-300.

About the author (1995)

Carl A.K. Borrebaeck is at University of Lund.

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