Carl A. K. Borrebaeck
Oxford University Press, 1995 - Medical - 390 pages
As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.
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Antibody Structure and Function
Human Monoclonal Antibodies from VGene Repertoires
Engineering the Antibody Combining Site by CodonBased
Human Metaphoric Antibodies from a Phagemid Library
Stragies for Humanizing Antibodies
SingleChain Fv Design and Production by Preparative Folding
Expressing Antibodies in Escherichia Coll
Antibodies and Antibody Fusion Proteins
The Cloning of Hybridoma V Regions for Their Ectopic
Working Examples 345 Working Examples
The Integrated Vector System
The Expression of Single Chain Antibodies
Acad activity added addition affinity allow amino acid amplified antibody fragments antigen approach assembly bacterial bacteriophage binding buffer cDNA cells chromatography cloning coli combining concentration conformation constant construct containing culture cytoplasmic described designed determined digested direct display disulfide domains engineering example expression Figure folding framework functional fusion protein gene glucose heavy chain human hybridoma immune immunoglobulin Incubate indicated isolated leader light chain linker loops method minutes molecules monoclonal antibody mouse murine obtained peptide periplasmic phage phagemid plasmid plate polymerase position possible prepared primers Proc production promoter protein Protocol purification reaction recombinant refolding regions remove repertoire residues restriction result scFv secreted selection sequence shown signal solution specificity step structure supernatant surface Table temperature variable regions vector volume Wash
Page 358 - Gossen, M. and Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.