Antibody Engineering

Front Cover
Carl A. K. Borrebaeck
Oxford University Press, 1995 - Medical - 390 pages
As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.

From inside the book

Contents

Antibody Structure and Function
3
AntibodyAntigen Interactions
39
Human Monoclonal Antibodies from VGene Repertoires
53
Creation of scFv Antibody Fragment Gene Repertoires
61
Generation of Human scFv Gene Repertoires
70
Conclusions
76
Selection of Phage Antibodies on Immobilized Antigen
82
Synthetic Antibodies
89
104
222
Expressing Antibodies in Escherichia Coll
229
Vectors and Approaches for the Eukaryotic Expression
267
107
277
Vectors for the Expression of IgFusion Proteins
280
The Cloning of Hybridoma V Regions for Their Ectopic
295
110
326
Ectopic Expression of Cloned V Regions for Intracellular and Intercellular
330

Engineering the Antibody Combining Site by CodonBased
117
Human Metaphoric Antibodies from a Phagemid Library
133
Stragies for Humanizing Antibodies
159
90
170
93
177
SingleChain Fv Design and Production by Preparative Folding
185
Working Examples
345
The Integrated Vector System
353
The Expression of Single Chain Antibodies
363
117
377
123
385
Copyright

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About the author (1995)

Carl A.K. Borrebaeck is at University of Lund.

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