Antibody EngineeringCarl A. K. Borrebaeck As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition--written by leading experts and now thoroughly updated--is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry. |
From inside the book
Results 1-5 of 78
Page ix
... Proteins Purification of Native sFv Protein Methodology for sFv Protein Production 186 192 193 201 205 Construction of sFv Genes 205 Cytoplasmic Expression of sFv Inclusion Bodies 207 Refolding Protocols for Cytoplasmic Inclusion Bodies ...
... Proteins Purification of Native sFv Protein Methodology for sFv Protein Production 186 192 193 201 205 Construction of sFv Genes 205 Cytoplasmic Expression of sFv Inclusion Bodies 207 Refolding Protocols for Cytoplasmic Inclusion Bodies ...
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Contents
Antibody Structure and Function | 3 |
AntibodyAntigen Interactions | 39 |
Human Monoclonal Antibodies from VGene Repertoires | 53 |
Creation of scFv Antibody Fragment Gene Repertoires | 61 |
Generation of Human scFv Gene Repertoires | 70 |
Conclusions | 76 |
Selection of Phage Antibodies on Immobilized Antigen | 82 |
Synthetic Antibodies | 89 |
104 | 222 |
Expressing Antibodies in Escherichia Coll | 229 |
Vectors and Approaches for the Eukaryotic Expression | 267 |
107 | 277 |
Vectors for the Expression of IgFusion Proteins | 280 |
The Cloning of Hybridoma V Regions for Their Ectopic | 295 |
110 | 326 |
Ectopic Expression of Cloned V Regions for Intracellular and Intercellular | 330 |
Engineering the Antibody Combining Site by CodonBased | 117 |
Human Metaphoric Antibodies from a Phagemid Library | 133 |
Stragies for Humanizing Antibodies | 159 |
90 | 170 |
93 | 177 |
SingleChain Fv Design and Production by Preparative Folding | 185 |
Common terms and phrases
Acad amino acid amplified antibody fragments antibody genes antigen antigen-binding bacterial BamHI binding Biol buffer cDNA cells centrifugation cloning codon constant region containing cytoplasmic denatured dilution disulfide bonds EcoRI EcoRV EDTA eluted encoding enzymes Escherichia coli ethanol expression vector Fab fragments filter folding functional fusion protein gene repertoires GuHCl heavy chain HindIII human antibodies hybridoma immunization immunoglobulin inclusion bodies Incubate intracellular lambda leader sequence ligation light chain linker method molecular molecules monoclonal antibody mouse mRNA murine mutagenesis N-terminal Natl oocytes overnight pellet peptide periplasmic phage display phagemid plasmid plate Plückthun polymerase Proc production promoter Protocol purification reaction recombinant refolding residues restriction sites resuspend room temperature scFv scFv fragments secreted selection sFv protein signal single-chain Fv soluble specific structure supernatant SurfZAP targeting transfection transgenic Tris-HCl V₁ variable domains variable regions Wash µg/ml
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