Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 2
After the salts have dissolved , adjust the volume of the solution to 100 ml with
deionized H , O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on
liquid cycle . ) SOB Medium Per liter : To 950 ml of deionized H , O , add : bacto ...
After the salts have dissolved , adjust the volume of the solution to 100 ml with
deionized H , O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on
liquid cycle . ) SOB Medium Per liter : To 950 ml of deionized H , O , add : bacto ...
Page 10
Adjust the pH to 7 . 0 with 0 . 1 n NaOH . Adjust the volume to 1 ml with distilled H
, 0 . Dispense the solution into small aliquots and store at - 70°C . Dissolve 770 g
of ammonium acetate in 800 ml of H , O . Adjust the volume to 1 liter with H , O ...
Adjust the pH to 7 . 0 with 0 . 1 n NaOH . Adjust the volume to 1 ml with distilled H
, 0 . Dispense the solution into small aliquots and store at - 70°C . Dissolve 770 g
of ammonium acetate in 800 ml of H , O . Adjust the volume to 1 liter with H , O ...
Page 13
Adjust the pH to 7 . 5 with 2 m acetic acid . Add pure H2O to 100 ml . Divide the
solution into aliquots and store them at - 20°C . To 60 ml of 5 m potassium
acetate , add 11 . 5 ml of glacial acetic acid and 28 . 5 ml of H , 0 . The resulting
solution ...
Adjust the pH to 7 . 5 with 2 m acetic acid . Add pure H2O to 100 ml . Divide the
solution into aliquots and store them at - 20°C . To 60 ml of 5 m potassium
acetate , add 11 . 5 ml of glacial acetic acid and 28 . 5 ml of H , 0 . The resulting
solution ...
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LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
BOOK | 3 |
Identification and Analysis of Recombinants 2 108 | 30 |
TRANSFECTION MEDIATED BY DEAEDEXTRAN 16 | 41 |
Copyright | |
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Common terms and phrases
acetate activity added Adjust aliquots allow amount antibody antigen Appendix appropriate approximately aqueous assay bacterial bacteriophage base binding buffer carried cells centrifugation Chapter chromatography Cl pH cloned coli column complete concentration containing culture deionized water described desired detected dilution Dissolve EDTA efficiency electrophoresis enzyme et al ethanol expression extract Figure filter final fragment gene growth hybridization Incubate levels ligation mammalian cells medium method mg/ml microfuge tube minutes mixture mRNAs nucleic acid pellet phase phosphate Place plasmid plate polymerase precipitation prepared procedure Products promoter protein radioactive radiolabeled reaction remove restriction room temperature sample screen selection sequence sodium solution specific step sterile stored strain TABLE Telephone termini tion transcription transfection transfer transformation Tris ug/ml usually vectors volume wash western blotting ОН
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Book 3 Molecular Cloning: A Laboratory Manual, Joseph Sambrook, ISBN 0879693096, 9780879693091 |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Feb 2010 |
| ISBN | 0879693096, 9780879693091 |
| Length | 412 pages |
| Export Citation | BiBTeX EndNote RefMan |