Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 52
Page xii
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
Page F-2
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ...
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ...
Page F-2
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ... DNA polymerase F.2 Subcloning FILLING RECESSED 3' TERMINI F 2.
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends on ... DNA polymerase F.2 Subcloning FILLING RECESSED 3' TERMINI F 2.
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml