Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 36
Page 18-31
... discarded after use . b Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract ... Discard contaminated clothing . PMSF is inactivated in aqueous solutions . The rate of inactivation increases with ...
... discarded after use . b Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract ... Discard contaminated clothing . PMSF is inactivated in aqueous solutions . The rate of inactivation increases with ...
Page 18-36
... Discard the supernatant ( unless the protein of interest is a secretory protein ) . 2. Resuspend the cells by vortexing in ice - cold phosphate - buffered saline ( PBS ; see Appendix B ) . Recover the cells by centrifugation as ...
... Discard the supernatant ( unless the protein of interest is a secretory protein ) . 2. Resuspend the cells by vortexing in ice - cold phosphate - buffered saline ( PBS ; see Appendix B ) . Recover the cells by centrifugation as ...
Page 18-56
... Discard the fixing solution , and add at least 5 gel volumes of 30 % ethanol . Incubate the gel for 30 minutes at room temperature with gentle shaking . 3. Repeat step 2 . 4. Discard the ethanol and add 10 gel volumes of deionized water ...
... Discard the fixing solution , and add at least 5 gel volumes of 30 % ethanol . Incubate the gel for 30 minutes at room temperature with gentle shaking . 3. Repeat step 2 . 4. Discard the ethanol and add 10 gel volumes of deionized water ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml