Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 35
Page B-9
... Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with H2O . Sterilize the solution by ...
... Dissolve 29 g of acrylamide and 1 g of N , N'- methylenebisacrylamide in a total volume of 60 ml of H2O . Heat the solution to 37 ° C to dissolve the chemicals . Adjust the volume to 100 ml with H2O . Sterilize the solution by ...
Page B-10
... Dissolve 60 mg of ATP in 0.8 ml of H2O . Adjust the pH to 7.0 with 0.1 N NaOH . Adjust the volume to 1 ml with distilled H2O . Dispense the solution into small aliquots and store at -70 ° C . Dissolve 770 g of ammonium acetate in 800 ml ...
... Dissolve 60 mg of ATP in 0.8 ml of H2O . Adjust the pH to 7.0 with 0.1 N NaOH . Adjust the volume to 1 ml with distilled H2O . Dispense the solution into small aliquots and store at -70 ° C . Dissolve 770 g of ammonium acetate in 800 ml ...
Page B-13
... Dissolve 9.82 g of potassium acetate in 90 ml of pure H2O ( Milli - Q or equivalent ) . Adjust the pH to 7.5 with 2 м acetic acid . Add pure H2O to 100 ml . Divide the solution into aliquots and store them at -20 ° C . To 60 ml of 5 м ...
... Dissolve 9.82 g of potassium acetate in 90 ml of pure H2O ( Milli - Q or equivalent ) . Adjust the pH to 7.5 with 2 м acetic acid . Add pure H2O to 100 ml . Divide the solution into aliquots and store them at -20 ° C . To 60 ml of 5 м ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml