Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 36
Page B-23
... EDTA 1 × : 0.09 M Tris - phosphate 0.002 M EDTA Tris - borate ( TBE ) Alkaline b 0.5x : 0.045 м Tris - borate 0.001 M EDTA 1x : 50 mn NaOH 1 mM EDTA Tris - glycine 1x : 25 mM Tris 250 mM glycine 0.1 % SDS Concentrated stock solution ...
... EDTA 1 × : 0.09 M Tris - phosphate 0.002 M EDTA Tris - borate ( TBE ) Alkaline b 0.5x : 0.045 м Tris - borate 0.001 M EDTA 1x : 50 mn NaOH 1 mM EDTA Tris - glycine 1x : 25 mM Tris 250 mM glycine 0.1 % SDS Concentrated stock solution ...
Page E-39
... EDTA ( pH 8.0 ) . 3. Rinse the tubing thoroughly in distilled water . 4. Boil for 10 minutes in 1 mM EDTA ( pH 8.0 ) . 5. Allow the tubing to cool , and then store it at 4 ° C . Be sure that the tubing is always submerged . From this ...
... EDTA ( pH 8.0 ) . 3. Rinse the tubing thoroughly in distilled water . 4. Boil for 10 minutes in 1 mM EDTA ( pH 8.0 ) . 5. Allow the tubing to cool , and then store it at 4 ° C . Be sure that the tubing is always submerged . From this ...
Page 1-44
... EDTA ) , preparation of buffer , B.20 TEACI . See Tetraethylammonium chloride TEMED ( N , N , N ' , N ' - tetramethylethyl- enediamine ) , use in polyacryl- amide gel electrophoresis , 6.36 , 18.49 TEN buffer for ion - exchange method ...
... EDTA ) , preparation of buffer , B.20 TEACI . See Tetraethylammonium chloride TEMED ( N , N , N ' , N ' - tetramethylethyl- enediamine ) , use in polyacryl- amide gel electrophoresis , 6.36 , 18.49 TEN buffer for ion - exchange method ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml