Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 58
Page 16-49
... Incubate the suspension for 5 minutes at 4 ° C . Lysozyme will not work efficiently if the pH of the solution is less than 8.0 . 4. Add 1 ml of ice - cold 0.25 m EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . · 5. Slowly add 1 ml of ...
... Incubate the suspension for 5 minutes at 4 ° C . Lysozyme will not work efficiently if the pH of the solution is less than 8.0 . 4. Add 1 ml of ice - cold 0.25 m EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . · 5. Slowly add 1 ml of ...
Page 17-19
... Incubate the mixture for 5 minutes at 70 ° C to denature the DNAs . c . Slowly cool to 18 ° C . d . Add 10 μl of 2 x elongation mix and 2 μl of the Klenow fragment of E. coli DNA polymerase I. Incubate for 30 minutes at room temperature ...
... Incubate the mixture for 5 minutes at 70 ° C to denature the DNAs . c . Slowly cool to 18 ° C . d . Add 10 μl of 2 x elongation mix and 2 μl of the Klenow fragment of E. coli DNA polymerase I. Incubate for 30 minutes at room temperature ...
Page 18-56
... Incubate the gel for 30 minutes at room temperature with gentle shaking . 3. Repeat step 2 . 4. Discard the ethanol and add 10 gel volumes of deionized water . Incubate the gel for 10 minutes at room temperature with gentle shaking . 5 ...
... Incubate the gel for 30 minutes at room temperature with gentle shaking . 3. Repeat step 2 . 4. Discard the ethanol and add 10 gel volumes of deionized water . Incubate the gel for 10 minutes at room temperature with gentle shaking . 5 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml