Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 33
Page 18-32
... NaCl 0.02 % sodium azide 0.1 % SDS 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 ...
... NaCl 0.02 % sodium azide 0.1 % SDS 100 μg / ml phenylmethylsulfonyl fluoride ( PMSF ) 1 μg / ml aprotinin 1 % Nonidet P - 40 ( NP - 40 ) 0.5 % sodium deoxycholate Single - detergent lysis buffer 50 mM Tris Cl ( pH 8.0 ) 150 mM NaCl 0.02 ...
Page B-13
... NaCl 10 % Sodium dodecyl sulfate ( SDS ) ( also called sodium lauryl sulfate ) 20 × SSC 20 × SSPE Trichloroacetic acid ( TCA ) 100 % solution 1 M Tris Dissolve 9.82 g of potassium acetate in 90 ml of pure H2O ( Milli - Q or equivalent ) ...
... NaCl 10 % Sodium dodecyl sulfate ( SDS ) ( also called sodium lauryl sulfate ) 20 × SSC 20 × SSPE Trichloroacetic acid ( TCA ) 100 % solution 1 M Tris Dissolve 9.82 g of potassium acetate in 90 ml of pure H2O ( Milli - Q or equivalent ) ...
Page B-28
... NaCl 60 mM CaCl2 60 mM MgCl2 100 mM Tris Cl ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 10 × Nuclease - S1 buffer 2 M NaCl 0.5 M sodium acetate ( pH 4.5 ) 10 mм ZnSO 5 % glycerol 10 × Mung - bean nuclease buffer 300 mM sodium acetate ( pH 4.5 ) 500 mM ...
... NaCl 60 mM CaCl2 60 mM MgCl2 100 mM Tris Cl ( pH 8.0 ) 1 mM EDTA ( pH 8.0 ) 10 × Nuclease - S1 buffer 2 M NaCl 0.5 M sodium acetate ( pH 4.5 ) 10 mм ZnSO 5 % glycerol 10 × Mung - bean nuclease buffer 300 mM sodium acetate ( pH 4.5 ) 500 mM ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml