Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 10
Page 16-12
... Sall 1700 SV40 2000 AAAA Intron 4000 SV40 BamHI 2740 Sall 3000 FIGURE 16.1D pko - neo expresses aminoglycoside phosphotransferase encoded by the bacterial trans- poson gene Tn5 neo from the eukaryotic SV40 early promoter ( PE ) or the ...
... Sall 1700 SV40 2000 AAAA Intron 4000 SV40 BamHI 2740 Sall 3000 FIGURE 16.1D pko - neo expresses aminoglycoside phosphotransferase encoded by the bacterial trans- poson gene Tn5 neo from the eukaryotic SV40 early promoter ( PE ) or the ...
Page 16-21
... Sall Xhol SV40 small Nhel Intron ori Io 5000 MMTV LTR PMSG ( 7.63 kb ) 2000 PE AAAA Bgill 2430 SV40 PLTR HindIII 2600 orl SV40 polyA BamHI 2940 SV40 small 3000 T intron 4000 Polycloning site FIGURE 16.3A PMSG is a transient expression ...
... Sall Xhol SV40 small Nhel Intron ori Io 5000 MMTV LTR PMSG ( 7.63 kb ) 2000 PE AAAA Bgill 2430 SV40 PLTR HindIII 2600 orl SV40 polyA BamHI 2940 SV40 small 3000 T intron 4000 Polycloning site FIGURE 16.3A PMSG is a transient expression ...
Page 17-5
... Sall Xbal Hindill Clal PUR288 TGT CGG GGA TCC GTC GAC TCT AGA AAG CTT ATC GAT GAT JL BamHI Sall JL JU Xbal Hindill Clal PUR289 TGT CAG GGG ATC CGT CGA CTC TAG AAA GCT TAT CGA TGA BamHI Sall Xbal Hindill Clal PUR290 TGT CAA AAA GGG GAT ...
... Sall Xbal Hindill Clal PUR288 TGT CGG GGA TCC GTC GAC TCT AGA AAG CTT ATC GAT GAT JL BamHI Sall JL JU Xbal Hindill Clal PUR289 TGT CAG GGG ATC CGT CGA CTC TAG AAA GCT TAT CGA TGA BamHI Sall Xbal Hindill Clal PUR290 TGT CAA AAA GGG GAT ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml