Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 49
Page 16-33
... Store in 5 - ml aliquots at -20 ° C . 2 M CaCl2 Dissolve 10.8 g of CaCl2 · 6H2O in 20 ml of distilled H2O . Sterilize the solution by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . 0.1 × TE ( pH 8.0 ) · 1 ...
... Store in 5 - ml aliquots at -20 ° C . 2 M CaCl2 Dissolve 10.8 g of CaCl2 · 6H2O in 20 ml of distilled H2O . Sterilize the solution by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . 0.1 × TE ( pH 8.0 ) · 1 ...
Page 18-15
... Store the suspension on ice for 1 hour . 3. Centrifuge the suspension at 10,000g for 10 minutes at 4 ° C . Discard the supernatant , and resuspend the pellet in acetone ( -20 ° C ) using the same amount that was used in step 2. Vigorous ...
... Store the suspension on ice for 1 hour . 3. Centrifuge the suspension at 10,000g for 10 minutes at 4 ° C . Discard the supernatant , and resuspend the pellet in acetone ( -20 ° C ) using the same amount that was used in step 2. Vigorous ...
Page B-10
... store at -70 ° C . Dissolve 770 g of ammonium acetate in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Sterilize by filtration . To 1 g of ammonium persulfate , add H2O to 10 ml . The solution may be stored for several weeks ...
... store at -70 ° C . Dissolve 770 g of ammonium acetate in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Sterilize by filtration . To 1 g of ammonium persulfate , add H2O to 10 ml . The solution may be stored for several weeks ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml