Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 86
Page xviii
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 16-63
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
Page 18-66
... transfer apparatus from the top downward , peeling off each layer in turn . Transfer the gel to a tray containing Coomassie Brilliant Blue , and stain it as described on page 18.55 . This will allow you to check whether electrophoretic ...
... transfer apparatus from the top downward , peeling off each layer in turn . Transfer the gel to a tray containing Coomassie Brilliant Blue , and stain it as described on page 18.55 . This will allow you to check whether electrophoretic ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml