Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 87
Page B-2
... acid , glacial Acetic acid Formic acid CH3COOH 60.05 17.4 1045 99.5 1.05 57.5 60.05 6.27 376 36 1.045 159.5 HCOOH 46.02 23.4 1080 90 1.20 42.7 Hydrochloric acid HCl 36.5 11.6 424 36 1.18 86.2 2.9 105 10 1.05 344.8 Nitric acid HNO3 63.02 ...
... acid , glacial Acetic acid Formic acid CH3COOH 60.05 17.4 1045 99.5 1.05 57.5 60.05 6.27 376 36 1.045 159.5 HCOOH 46.02 23.4 1080 90 1.20 42.7 Hydrochloric acid HCl 36.5 11.6 424 36 1.18 86.2 2.9 105 10 1.05 344.8 Nitric acid HNO3 63.02 ...
Page E-16
... acid solution can be reduced significantly . This method of concentration is used to reduce the volume of dilute solutions to the point where the nucleic acid can easily be recovered by precipitation with ethanol . 1. Measure the volume ...
... acid solution can be reduced significantly . This method of concentration is used to reduce the volume of dilute solutions to the point where the nucleic acid can easily be recovered by precipitation with ethanol . 1. Measure the volume ...
Page E-18
... acid products with trichloroacetic acid ( TCA ) and ( 2 ) differential adsorption of the products onto positively charged surfaces ( e.g. , DE - 81 paper ) . Precipitation of Nucleic Acids with Trichloroacetic Acid 1. Using a soft ...
... acid products with trichloroacetic acid ( TCA ) and ( 2 ) differential adsorption of the products onto positively charged surfaces ( e.g. , DE - 81 paper ) . Precipitation of Nucleic Acids with Trichloroacetic Acid 1. Using a soft ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml