Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-57
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
Page 16-58
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a ...
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a ...
Page 18-2
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml