Molecular cloning: a laboratory manual, Volume 3 |
What people are saying - Write a review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Other editions - View all
Common terms and phrases
acetate acrylamide activity Adjust the volume aliquots amino acids ammonium persulfate amount antigen antisera aqueous assay autoclaving bacteria bacteriophage T7 binding bovine serum albumin cDNA cell lines cloned gene coli column culture deionized H2O denatured described on pages dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution Escherichia coli ethanol eukaryotic expression extract foreign protein fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate lysate lysis buffer mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture monoclonal antibodies mRNA NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyclonal precipitate prepared promoter protein of interest radioactive radiolabeled reaction reagent Remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequence sodium sodium azide sterile stock solution strain supernatant target antigen target protein termini transcription transfection Transfer Tris Cl pH vectors wash western blotting
Bibliographic information
| Title | Molecular cloning: a laboratory manual, Volume 3 Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091 |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Feb 2010 |
| ISBN | 0879693096, 9780879693091 |
| Subjects | Eukaryotic cells Molecular cloning |
| Export Citation | BiBTeX EndNote RefMan |