Molecular Cloning: A Laboratory Manual, Book 3 |
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Page F-2
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
Page F-2
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
... added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) ... added to a final concentration of 5 mM before the appropriate dNTPs and the Klenow fragment of E. coli DNA ...
Page F-10
... added linkers to a fragment that carries compatible termini . Note The effectiveness of the restriction enzyme digestion can be tested as follows : a . Before adding the enzyme in step 3 , remove 2 μl from the reaction and store at 4 ...
... added linkers to a fragment that carries compatible termini . Note The effectiveness of the restriction enzyme digestion can be tested as follows : a . Before adding the enzyme in step 3 , remove 2 μl from the reaction and store at 4 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml