Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 37
Page 16-33
... aliquots at -20 ° C . 2 M CaCl2 Dissolve 10.8 g of CaCl2 · 6H2O in 20 ml of distilled H2O . Sterilize the solution by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . 0.1 × TE ( pH 8.0 ) · 1 mM Tris Cl ( pH ...
... aliquots at -20 ° C . 2 M CaCl2 Dissolve 10.8 g of CaCl2 · 6H2O in 20 ml of distilled H2O . Sterilize the solution by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . 0.1 × TE ( pH 8.0 ) · 1 mM Tris Cl ( pH ...
Page B-10
... aliquots at -20 ° C . Dissolve 54 g of CaCl2 6H2O in 200 ml of pure H2O ( Milli - Q or equivalent ) . Sterilize the solu- tion by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . Dissolve 13.5 g of CaCl2 ...
... aliquots at -20 ° C . Dissolve 54 g of CaCl2 6H2O in 200 ml of pure H2O ( Milli - Q or equivalent ) . Sterilize the solu- tion by passage through a 0.22 - micron filter . Store in 1 - ml aliquots at -20 ° C . Dissolve 13.5 g of CaCl2 ...
Page B-13
... aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ...
... aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve 100 g of electrophoresis - grade SDS in 900 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml