Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 57
Page 17-31
... allow secretion of a given foreign protein . Even then , processing may not occur in the correct position and protein levels may be low . One system that may allow secretion and processing takes advantage of the alkaline phosphatase ...
... allow secretion of a given foreign protein . Even then , processing may not occur in the correct position and protein levels may be low . One system that may allow secretion and processing takes advantage of the alkaline phosphatase ...
Page 17-34
... allow the protein of interest to be expressed in E. coli are constructed , the amount of protein made is measured . Generally , four approaches are used : 1. SDS - polyacrylamide gels are run to determine whether a protein of the ...
... allow the protein of interest to be expressed in E. coli are constructed , the amount of protein made is measured . Generally , four approaches are used : 1. SDS - polyacrylamide gels are run to determine whether a protein of the ...
Page E-21
... allow the emitted ẞ particles to interact productively with silver halide crystals in the emulsion , it is not enough to allow the particles to pass through barriers ( e.g. , Saran Wrap ) that might be placed between the film and the ...
... allow the emitted ẞ particles to interact productively with silver halide crystals in the emulsion , it is not enough to allow the particles to pass through barriers ( e.g. , Saran Wrap ) that might be placed between the film and the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml