Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-19
... amount of target antigens . These assays include immunoprecipitation , western blotting , and solid - phase radioimmunoassays ( RIAs ) , which will be described in detail in the remain- der of this chapter . SOLID - PHASE ...
... amount of target antigens . These assays include immunoprecipitation , western blotting , and solid - phase radioimmunoassays ( RIAs ) , which will be described in detail in the remain- der of this chapter . SOLID - PHASE ...
Page 18-21
... amount of the radiolabeled second antibody that reacts with antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized ...
... amount of the radiolabeled second antibody that reacts with antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized ...
Page E-5
... amount of nucleic acid in a preparation . If the sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ...
... amount of nucleic acid in a preparation . If the sample is pure ( i.e. , without significant amounts of contaminants such as proteins , phenol , agarose , or other nucleic acids ) , spectrophotometric measurement of the amount of ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml