Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-4
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
Page 18-5
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
Page 18-21
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 10 ...
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 10 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml