Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 36
Page 18-12
... approximately 10-20 mg of IgG ( equivalent to 1-2 ml of antiserum ) . Antibodies bind to protein A chiefly by hydrophobic interactions ( Deisenhofer 1981 ) that can be disrupted at low pH . Protein A is remarkably resilient and ...
... approximately 10-20 mg of IgG ( equivalent to 1-2 ml of antiserum ) . Antibodies bind to protein A chiefly by hydrophobic interactions ( Deisenhofer 1981 ) that can be disrupted at low pH . Protein A is remarkably resilient and ...
Page 18-43
... approximately 10 mg / ml . Super- natants from cultured hybridoma cells typically contain 10-100 μg / ml immuno- globulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 2. Incubate the mixtures for 1 hour at 0 ° C . 3 ...
... approximately 10 mg / ml . Super- natants from cultured hybridoma cells typically contain 10-100 μg / ml immuno- globulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 2. Incubate the mixtures for 1 hour at 0 ° C . 3 ...
Page 18-79
... Approximately 70 mM KCl is usually optimal for translation of capped mRNAs . Approximately 40 mM KCl is usually optimal for translation of uncapped mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA ...
... Approximately 70 mM KCl is usually optimal for translation of capped mRNAs . Approximately 40 mM KCl is usually optimal for translation of uncapped mRNAs . Up to 10 μg of total cytoplasmic RNA or 0.2 μg of poly ( A ) * mRNA or RNA ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml