Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 32
PMSF , which is labile in aqueous solution , should be added from a stock
solution just before the lysis buffer is used . The rate of inactivation in aqueous
solution increases with pH and is faster at 25°C than at 4°C . The half - life of a 20
um ...
PMSF , which is labile in aqueous solution , should be added from a stock
solution just before the lysis buffer is used . The rate of inactivation in aqueous
solution increases with pH and is faster at 25°C than at 4°C . The half - life of a 20
um ...
Page 62
PMSF , which is labile in aqueous solutions , should be added from a stock
solution just before the suspension buffer is used . The rate of inactivation in
aqueous solution increases with pH and is faster at 25°C than at 4°C . The half -
life of a ...
PMSF , which is labile in aqueous solutions , should be added from a stock
solution just before the suspension buffer is used . The rate of inactivation in
aqueous solution increases with pH and is faster at 25°C than at 4°C . The half -
life of a ...
Page 3
The key step , the removal of proteins , can often be carried out simply by
extracting aqueous solutions of nucleic acids with phenol : chloroform and
chloroform . Such extractions are used whenever it is necessary to inactivate and
remove ...
The key step , the removal of proteins , can often be carried out simply by
extracting aqueous solutions of nucleic acids with phenol : chloroform and
chloroform . Such extractions are used whenever it is necessary to inactivate and
remove ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
Cosmid Vectors | 3 |
Introduction of Recombinant Vectors into Mammalian Cells 16 | 30 |
TRANSFECTION MEDIATED BY DEAEDEXTRAN 16 | 41 |
Copyright | |
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Common terms and phrases
acetate acid activity added Adjust aliquots allow amount antibody antigen Appendix appropriate approximately assay bacteria bacteriophage binding buffer carried cells centrifugation Cl pH cloned coli column complete concentration containing culture deionized water described desired detected determined dilution Dissolve efficiency electrophoresis enzyme et al ethanol expression extract Figure filter final foreign fragment gene growth increase Incubate levels lines mammalian cells medium method mg/ml microfuge minutes mixture mRNA nucleic acid obtained pellet Place plasmid plate polymerase precipitate prepared presence production promoter protein radioactive radiolabeled reaction Remove restriction room temperature sample selection sequences sodium solution specific step sterile stored strain supernatant TABLE target protein Telephone termini tion transcription transfected Transfer transformation translation Tris tube ug/ml usually vectors volume wash western blotting ОН