Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 54
Page 16-58
... assay . The CAT activity is then normalized to the B - galactosidase activity . Alternatively , the ẞ - galactosidase activity in a constant volume of extract is measured and the CAT assay is then carried out with amounts of extract ...
... assay . The CAT activity is then normalized to the B - galactosidase activity . Alternatively , the ẞ - galactosidase activity in a constant volume of extract is measured and the CAT assay is then carried out with amounts of extract ...
Page 16-66
... assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for 10 minutes after the B - galactosidase assays are completed . ii . It is essential to include positive and negative controls of the ...
... assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for 10 minutes after the B - galactosidase assays are completed . ii . It is essential to include positive and negative controls of the ...
Page 18-19
... assay can be extremely sensitive but requires that target protein is available ( prefer- ably in a pure form ) to serve both as a competitor and as a standard . • Immobilized antigen RIAs : In this method , unlabeled antigen is attached ...
... assay can be extremely sensitive but requires that target protein is available ( prefer- ably in a pure form ) to serve both as a competitor and as a standard . • Immobilized antigen RIAs : In this method , unlabeled antigen is attached ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml