Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-49
... base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted with HCl as described in Appendix B. If Tris Cl or Trizma is used to prepare buffers , the concentration of salt will be too ...
... base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted with HCl as described in Appendix B. If Tris Cl or Trizma is used to prepare buffers , the concentration of salt will be too ...
Page C-2
... base normals to helical axis = -1.2 ° Propeller twist of base pair ( mean ) = + 16 ° Number of base pairs per helical turn = -10 3.32 Å rise per base pair FIGURE C.1 The B form of DNA is a right - handed helix , whose axis passes through ...
... base normals to helical axis = -1.2 ° Propeller twist of base pair ( mean ) = + 16 ° Number of base pairs per helical turn = -10 3.32 Å rise per base pair FIGURE C.1 The B form of DNA is a right - handed helix , whose axis passes through ...
Page C-13
... base cytosine base = guanine base = thymine Molecular weight 451.2 491.2 466.2 ; Na , H2O , 608.2 TABLE D.2 Properties of Amino Acids Three - letter Amino Properties of Nucleic Acids C.13 Nucleoside Analogs Used as Chain Terminators in ...
... base cytosine base = guanine base = thymine Molecular weight 451.2 491.2 466.2 ; Na , H2O , 608.2 TABLE D.2 Properties of Amino Acids Three - letter Amino Properties of Nucleic Acids C.13 Nucleoside Analogs Used as Chain Terminators in ...
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acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml