Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-17
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
Page 18-11
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
Page 18-21
... binding sites on the surface of the solid support and , at saturation , each well of the plate binds approximately ... binding sites on the solid support . To maximize binding of relevant antibodies , immunoglobulins directed against the ...
... binding sites on the surface of the solid support and , at saturation , each well of the plate binds approximately ... binding sites on the solid support . To maximize binding of relevant antibodies , immunoglobulins directed against the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml