Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 44
Page xxxii
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.35 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.35 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
Page E-30
... Chromatography on hydroxyapatite can therefore be used to separate single - stranded DNA from double - stranded DNA or DNA : RNA hybrids . In molecular cloning , hydroxyapatite chromatography is chiefly used to prepare radiolabeled ...
... Chromatography on hydroxyapatite can therefore be used to separate single - stranded DNA from double - stranded DNA or DNA : RNA hybrids . In molecular cloning , hydroxyapatite chromatography is chiefly used to prepare radiolabeled ...
Page 1-7
... chromatography , 16.60-16.63 as reporter gene , 16.56 vectors , 16.57 4 - Chloro - 1 - naphthol , detection of antibody - horseradish peroxidase complexes , stock solution , 12.19 Chloroquine treatment following transfec- tion of ...
... chromatography , 16.60-16.63 as reporter gene , 16.56 vectors , 16.57 4 - Chloro - 1 - naphthol , detection of antibody - horseradish peroxidase complexes , stock solution , 12.19 Chloroquine treatment following transfec- tion of ...
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acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml