Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 15
Page xxvii
... Cleaved by a Protease or Cyanogen Bromide 17.27 PRODUCTION OF HYBRID PROTEINS THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 QUANTITATING THE LEVELS ...
... Cleaved by a Protease or Cyanogen Bromide 17.27 PRODUCTION OF HYBRID PROTEINS THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 QUANTITATING THE LEVELS ...
Page 17-29
... Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein composed of a prokaryotic amino terminus connected to the protein of interest by a se- quence that can be cleaved by a protease or by ...
... Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein composed of a prokaryotic amino terminus connected to the protein of interest by a se- quence that can be cleaved by a protease or by ...
Page 17-30
... CLEAVED WITH FACTOR X. The expression plasmid of Nagai and Thøgersen is constructed as follows : 1. Using one of the three methods described on pages 17.18-17.24 , prepare a cDNA fragment with a blunt 5 ' terminus immediately preceding ...
... CLEAVED WITH FACTOR X. The expression plasmid of Nagai and Thøgersen is constructed as follows : 1. Using one of the three methods described on pages 17.18-17.24 , prepare a cDNA fragment with a blunt 5 ' terminus immediately preceding ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml