Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-2
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells BOOK.
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells BOOK.
Page 16-2
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells BOOK.
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells BOOK.
Page 16-3
... CLONED GENES A few eukaryotic proteins have been expressed efficiently and inexpensively in prokaryotic hosts ( see Chapter 17 ) . However , many eukaryotic proteins synthesized in bacteria fold incorrectly or inefficiently and ...
... CLONED GENES A few eukaryotic proteins have been expressed efficiently and inexpensively in prokaryotic hosts ( see Chapter 17 ) . However , many eukaryotic proteins synthesized in bacteria fold incorrectly or inefficiently and ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml