Molecular Cloning: A Laboratory Manual, Book 3

acetate acid activity added Adjust aliquots allow amount antibody antigen Appendix appropriate approximately assay bacteria bacteriophage binding buffer carried cells centrifugation Cl pH cloned coli column complete concentration containing culture deionized water described desired detected determined dilution Dissolve efficiency electrophoresis enzyme et al ethanol expression extract Figure filter final foreign fragment gene growth increase Incubate levels lines mammalian cells medium method mg/ml microfuge minutes mixture mRNA nucleic acid obtained pellet Place plasmid plate polymerase precipitate prepared presence production promoter protein radioactive radiolabeled reaction Remove restriction room temperature sample selection sequences sodium solution specific step sterile stored strain supernatant TABLE target protein Telephone termini tion transcription transfected Transfer transformation translation Tris tube ug/ml usually vectors volume wash western blotting ОН