Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xiv
... Denaturation with Glyoxal and Dimethyl Sulfoxide 7.40 Electrophoresis of RNA through Gels Containing Formaldehyde 7.43 Transfer of Denatured RNA to Nitrocellulose Filters ... Denatured RNA to Nylon Membranes 7.49 Staininging RNA xiv Contents.
... Denaturation with Glyoxal and Dimethyl Sulfoxide 7.40 Electrophoresis of RNA through Gels Containing Formaldehyde 7.43 Transfer of Denatured RNA to Nitrocellulose Filters ... Denatured RNA to Nylon Membranes 7.49 Staininging RNA xiv Contents.
Page 18-3
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
Page 18-4
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml