Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xiv
... Denaturation with Glyoxal and Dimethyl Sulfoxide 7.40 Electrophoresis of RNA through Gels Containing Formaldehyde 7.43 Transfer of Denatured RNA to Nitrocellulose Filters ... Denatured RNA to Nylon Membranes 7.49 Staininging RNA xiv Contents.
... Denaturation with Glyoxal and Dimethyl Sulfoxide 7.40 Electrophoresis of RNA through Gels Containing Formaldehyde 7.43 Transfer of Denatured RNA to Nitrocellulose Filters ... Denatured RNA to Nylon Membranes 7.49 Staininging RNA xiv Contents.
Page 18-3
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
... denatured forms of the protein . These are raised against fully denatured antigens and can be either monoclonal or polyclonal . Factors Affecting Immune Response Although there is no way to guarantee the production of particular types ...
Page 18-4
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
... denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured protein . Many immunology textbooks state that denatured pro ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml