Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 85
Page 16-38
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
Page 17-25
... described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages 17.18- 17.24 will contain a ...
... described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages 17.18- 17.24 will contain a ...
Page E-26
... described in Chapter 6 , page 6.45 , and dried onto Whatman 3MM paper using a commercial gel dryer . b . Sequencing gels containing 35S should be fixed as described in Chapter 13 , page 13.56 , and dried onto Whatman 3MM paper using a ...
... described in Chapter 6 , page 6.45 , and dried onto Whatman 3MM paper using a commercial gel dryer . b . Sequencing gels containing 35S should be fixed as described in Chapter 13 , page 13.56 , and dried onto Whatman 3MM paper using a ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml