Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 57
Page 16-72
... determine sensitivity of the target gene to digestion with a particular enzyme . If the gene is not large , it is sometimes possible to prepare a genomic DNA library in a bacteriophage A or cosmid vector and use purified library DNA for ...
... determine sensitivity of the target gene to digestion with a particular enzyme . If the gene is not large , it is sometimes possible to prepare a genomic DNA library in a bacteriophage A or cosmid vector and use purified library DNA for ...
Page 17-8
... determined by plasmid minipreps , can be screened for protein expression later . cDNA inserts into a pMR100 plasmid can ... determine the time at which the maximum amount of product is produced . e . Resuspend each pellet in 100 μl of 1 ...
... determined by plasmid minipreps , can be screened for protein expression later . cDNA inserts into a pMR100 plasmid can ... determine the time at which the maximum amount of product is produced . e . Resuspend each pellet in 100 μl of 1 ...
Page 18-79
... determined for every new batch of reticulocyte lysate using a standard preparation of mRNA . Titrate the magnesium acetate over the range of 0 to 1 mM . Then , using the optimum concentration of magnesium acetate , determine the effect ...
... determined for every new batch of reticulocyte lysate using a standard preparation of mRNA . Titrate the magnesium acetate over the range of 0 to 1 mM . Then , using the optimum concentration of magnesium acetate , determine the effect ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml