Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-46
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
Page 16-54
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
Page 16-73
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml